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Image Search Results
Journal: Cell Communication and Signaling : CCS
Article Title: Peritoneal cavity-derived small extracellular vesicles from aged tumor-naïve hosts promote ovarian cancer adhesion and invasion
doi: 10.1186/s12964-025-02273-1
Figure Lengend Snippet: sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight NS300 nanoparticle tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Article Snippet: sEV sizes and concentrations were determined using the NanoSight
Techniques: Isolation, Membrane, Derivative Assay, Western Blot, Concentration Assay, Transmission Assay, Electron Microscopy