se v 16 software Search Results


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Malvern Panalytical nanoparticle tracking analysis nta instrument
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
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STATA Corporation stata/se v.16.0
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
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STATA Corporation se v 16 software
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Se V 16 Software, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Stata/Se V.16.1 For Mac, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Standard Statistical Software Stata/Se V.16, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STATA Corporation statistical software package v.16 se
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Statistical Software Package V.16 Se, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Bma Command In Stata/Se V.16.0, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STATA Corporation se v 16
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Se V 16, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Se V 16 1, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STATA Corporation meta-regressions
sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight <t>NS300</t> <t>nanoparticle</t> tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size
Meta Regressions, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight NS300 nanoparticle tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size

Journal: Cell Communication and Signaling : CCS

Article Title: Peritoneal cavity-derived small extracellular vesicles from aged tumor-naïve hosts promote ovarian cancer adhesion and invasion

doi: 10.1186/s12964-025-02273-1

Figure Lengend Snippet: sEV isolation and characterization. ( A ) Young (3–6 month) and aged (20–22 month) healthy mice ( n = 3/group) were subjected to peritoneal lavage once a week for 3 weeks. Lavage fluids from each mouse were pooled prior to sEV isolation. ( B ) sEV isolation was accomplished using a NanoEX™ system, a bionanoparticle isolation technology microfluidic cassette housing an asymmetric nanopore membrane, according to the manufacturer’s specifications. ( C ) sEVs derived from young and aged peritoneal lavage (20 ug) were electrophoresed on a 9% SDS polyacrylamide gel and western-blotted to PVDF membrane. Blots were probed with antibodies to CD63 and TSG101 at a 1:100 dilution, and with a peroxidase-conjugated rabbit secondary antibody at a 1:4000 dilution. ( D ) NanoSight NS300 nanoparticle tracking analysis was performed immediately following sEV isolation. sEVs typically had a mean size slightly larger than 100 nm with little large particle contaminants. Plots show nanoparticle size and concentration. ( E ) Transmission electron microscopy of sEVs from young and aged mouse lavages. Plot shows nanoparticle size

Article Snippet: sEV sizes and concentrations were determined using the NanoSight nanoparticle tracking analysis (NTA) instrument (NS300, Software Version: NTA 3.2 Dev Build 3.2.16; Malvern Panalytical, Malvern WR14 1XZ, UK).

Techniques: Isolation, Membrane, Derivative Assay, Western Blot, Concentration Assay, Transmission Assay, Electron Microscopy